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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
DDX39
QPCR Primer Pair List
: primer pair list
QPCR Relative Expression Quantification
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | NIH:OVCAR-3-Crashing;Lipofectamine 2000 |
| 2 | allstar2_s;NIH:OVCAR-3-Crashing |
| 3 | RBM38_Global_177_1_s;NIH:OVCAR-3-Crashing |
| 4 | RBM38_Global_1782_s;NIH:OVCAR-3-Crashing |
| 5 | SI_DDX17_1;NIH:OVCAR-3-Crashing |
| 6 | SI_DDX17_2;NIH:OVCAR-3-Crashing |
| 7 | siDDX39_global_1;NIH:OVCAR-3-Crashing |
| 8 | siDDX39_global_2;NIH:OVCAR-3-Crashing |
| 9 | siSF3A1 global_1;NIH:OVCAR-3-Crashing |
| 10 | siSF3A1 global_2;NIH:OVCAR-3-Crashing |
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Page 1 of 1, records 1 to 10 of 10
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DDX17_G_1_f DDX17_G_r | 1.000 | 0.993 | 0.670 | 0.741 | ||||||||||||||||
| DDX39_G_1_f DDX39_G_r | 1.000 | 0.986 | 0.537 | 0.342 | ||||||||||||||||
| RBM38_G_f RBM38_G_r | 1.000 | 0.991 | 0.632 | 0.362 | ||||||||||||||||
| SF3A1_G_4_f SF3A1_G_3_r | 1.000 | 0.865 | 0.359 | 0.469 |
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Page 1 of 1, records 1 to 4 of 4
QPCR Relative Expression Quantification
: Systematic Outlier Filtering
1 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
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0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
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QPCR Reference Gene Page
: .
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