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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
CWC25 CCDC94 LUC7L3 DDX42 HSPA2 LUC7L
QPCR Primer Pair List
: primer pair list
| Gene | Forward | Reverse | |
|---|---|---|---|
| VIEW | CCDC49 | CCDC49.G1.F1 | CCDC49.G1.R1 |
| VIEW | CCDC94 | CCDC94.G1.F1 | CCDC94.G1.R1 |
| VIEW | CROP | CROP_G_1_f | CROP_G_r |
| VIEW | DDX42 | DDX42_G_f | DDX42_G_r |
| VIEW | DHX38 | DHX38_G_f | DHX38_G_r |
| VIEW | HSPA2 | HSPA2_G_3_f | HSPA2_G_2_r |
| VIEW | LUC7L | LUC7L_G_1_f | LUC7L_G_r |
| VIEW | MBNL2 | MBNL2_G_1_f | MBNL2_G_1_r |
| VIEW | PSMC4 | PSMC4_G_1_f | PSMC4_G_1_r |
| VIEW | SDHA | SDHA_G_f | SDHA_G_r |
current - show all
Page 1 of 1, records 1 to 10 of 10
QPCR Relative Expression Quantification (N1-3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CCDC49__G_1_s;SKOV3ip1 |
| 2 | CCDC49__G_s;SKOV3ip1 |
| 3 | CCDC94__G_1_s;SKOV3ip1 |
| 4 | CCDC94__G_s;SKOV3ip1 |
| 5 | CROP__G_1_s;SKOV3ip1 |
| 6 | CROP__G_s;SKOV3ip1 |
| 7 | DDX42__G_1_s;SKOV3ip1 |
| 8 | DDX42__G_s;SKOV3ip1 |
| 9 | DHX38__G_s;SKOV3ip1 |
| 10 | HSPA2__G_1_s;SKOV3ip1 |
| 11 | HSPA2__G_s;SKOV3ip1 |
| 12 | KITLG_84_L_2_s;SKOV3ip1 |
| 13 | LUC7L__G_1_s;SKOV3ip1 |
| 14 | LUC7L__G_s;SKOV3ip1 |
| 15 | MBNL2__G_1_s;SKOV3ip1 |
| 16 | MBNL2__G_s;SKOV3ip1 |
| 17 | SKOV3ip1;Lipofectamine 2000 |
current - show all
Page 1 of 1, records 1 to 17 of 17
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCDC49.G1.F1 CCDC49.G1.R1 | 0.461 | 0.098 | 0.730 | 1.000 | ||||||||||||||||||||||||||||||
| CCDC94.G1.F1 CCDC94.G1.R1 | 0.317 | 0.065 | 0.859 | 1.000 | ||||||||||||||||||||||||||||||
| CROP_G_1_f CROP_G_r | 0.070 | 0.048 | 0.883 | 1.000 | ||||||||||||||||||||||||||||||
| DDX42_G_f DDX42_G_r | 0.078 | 0.072 | 0.856 | 1.000 | ||||||||||||||||||||||||||||||
| DHX38_G_f DHX38_G_r | 1.000 | 1.000 | ||||||||||||||||||||||||||||||||
| HSPA2_G_3_f HSPA2_G_2_r | 0.056 | 0.062 | 0.549 | 1.000 | ||||||||||||||||||||||||||||||
| LUC7L_G_1_f LUC7L_G_r | 1.102 | 0.307 | 0.405 | 1.000 | ||||||||||||||||||||||||||||||
| MBNL2_G_1_f MBNL2_G_1_r | 0.850 | 0.775 | 0.099 | 1.000 |
current - show all
Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification (N1)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CCDC49__G_1_s;SKOV3ip1 |
| 2 | CCDC49__G_s;SKOV3ip1 |
| 3 | CCDC94__G_1_s;SKOV3ip1 |
| 4 | CCDC94__G_s;SKOV3ip1 |
| 5 | CROP__G_1_s;SKOV3ip1 |
| 6 | CROP__G_s;SKOV3ip1 |
| 7 | DDX42__G_1_s;SKOV3ip1 |
| 8 | DDX42__G_s;SKOV3ip1 |
| 9 | DHX38__G_s;SKOV3ip1 |
| 10 | HSPA2__G_1_s;SKOV3ip1 |
| 11 | HSPA2__G_s;SKOV3ip1 |
| 12 | KITLG_84_L_2_s;SKOV3ip1 |
| 13 | LUC7L__G_1_s;SKOV3ip1 |
| 14 | LUC7L__G_s;SKOV3ip1 |
| 15 | MBNL2__G_1_s;SKOV3ip1 |
| 16 | MBNL2__G_s;SKOV3ip1 |
| 17 | SKOV3ip1;Lipofectamine 2000 |
current - show all
Page 1 of 1, records 1 to 17 of 17
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCDC49.G1.F1 CCDC49.G1.R1 | 0.461 | 0.098 | 0.730 | 1.000 | ||||||||||||||||||||||||||||||
| CCDC94.G1.F1 CCDC94.G1.R1 | 0.317 | 0.065 | 0.859 | 1.000 | ||||||||||||||||||||||||||||||
| CROP_G_1_f CROP_G_r | 0.070 | 0.048 | 0.883 | 1.000 | ||||||||||||||||||||||||||||||
| DDX42_G_f DDX42_G_r | 0.078 | 0.072 | 0.856 | 1.000 | ||||||||||||||||||||||||||||||
| DHX38_G_f DHX38_G_r | 1.000 | 1.000 | ||||||||||||||||||||||||||||||||
| HSPA2_G_3_f HSPA2_G_2_r | 0.056 | 0.062 | 0.549 | 1.000 | ||||||||||||||||||||||||||||||
| LUC7L_G_1_f LUC7L_G_r | 1.102 | 0.307 | 0.405 | 1.000 | ||||||||||||||||||||||||||||||
| MBNL2_G_1_f MBNL2_G_1_r | 0.850 | 0.775 | 0.099 | 1.000 |
current - show all
Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification (N2)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CCDC49__G_1_s;SKOV3ip1 |
| 2 | CCDC49__G_s;SKOV3ip1 |
| 3 | CCDC94__G_1_s;SKOV3ip1 |
| 4 | CCDC94__G_s;SKOV3ip1 |
| 5 | CROP__G_1_s;SKOV3ip1 |
| 6 | CROP__G_s;SKOV3ip1 |
| 7 | DDX42__G_1_s;SKOV3ip1 |
| 8 | DDX42__G_s;SKOV3ip1 |
| 9 | DHX38__G_s;SKOV3ip1 |
| 10 | HSPA2__G_1_s;SKOV3ip1 |
| 11 | HSPA2__G_s;SKOV3ip1 |
| 12 | KITLG_84_L_2_s;SKOV3ip1 |
| 13 | LUC7L__G_1_s;SKOV3ip1 |
| 14 | LUC7L__G_s;SKOV3ip1 |
| 15 | MBNL2__G_1_s;SKOV3ip1 |
| 16 | MBNL2__G_s;SKOV3ip1 |
| 17 | SKOV3ip1;Lipofectamine 2000 |
current - show all
Page 1 of 1, records 1 to 17 of 17
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCDC49.G1.F1 CCDC49.G1.R1 | 0.461 | 0.098 | 0.730 | 1.000 | ||||||||||||||||||||||||||||||
| CCDC94.G1.F1 CCDC94.G1.R1 | 0.317 | 0.065 | 0.859 | 1.000 | ||||||||||||||||||||||||||||||
| CROP_G_1_f CROP_G_r | 0.070 | 0.048 | 0.883 | 1.000 | ||||||||||||||||||||||||||||||
| DDX42_G_f DDX42_G_r | 0.078 | 0.072 | 0.856 | 1.000 | ||||||||||||||||||||||||||||||
| DHX38_G_f DHX38_G_r | 1.000 | 1.000 | ||||||||||||||||||||||||||||||||
| HSPA2_G_3_f HSPA2_G_2_r | 0.056 | 0.062 | 0.549 | 1.000 | ||||||||||||||||||||||||||||||
| LUC7L_G_1_f LUC7L_G_r | 1.102 | 0.307 | 0.405 | 1.000 | ||||||||||||||||||||||||||||||
| MBNL2_G_1_f MBNL2_G_1_r | 0.850 | 0.775 | 0.099 | 1.000 |
current - show all
Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification (N3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | CCDC49__G_1_s;SKOV3ip1 |
| 2 | CCDC49__G_s;SKOV3ip1 |
| 3 | CCDC94__G_1_s;SKOV3ip1 |
| 4 | CCDC94__G_s;SKOV3ip1 |
| 5 | CROP__G_1_s;SKOV3ip1 |
| 6 | CROP__G_s;SKOV3ip1 |
| 7 | DDX42__G_1_s;SKOV3ip1 |
| 8 | DDX42__G_s;SKOV3ip1 |
| 9 | DHX38__G_s;SKOV3ip1 |
| 10 | HSPA2__G_1_s;SKOV3ip1 |
| 11 | HSPA2__G_s;SKOV3ip1 |
| 12 | KITLG_84_L_2_s;SKOV3ip1 |
| 13 | LUC7L__G_1_s;SKOV3ip1 |
| 14 | LUC7L__G_s;SKOV3ip1 |
| 15 | MBNL2__G_1_s;SKOV3ip1 |
| 16 | MBNL2__G_s;SKOV3ip1 |
| 17 | SKOV3ip1;Lipofectamine 2000 |
current - show all
Page 1 of 1, records 1 to 17 of 17
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCDC49.G1.F1 CCDC49.G1.R1 | 0.461 | 0.098 | 0.730 | 1.000 | ||||||||||||||||||||||||||||||
| CCDC94.G1.F1 CCDC94.G1.R1 | 0.317 | 0.065 | 0.859 | 1.000 | ||||||||||||||||||||||||||||||
| CROP_G_1_f CROP_G_r | 0.070 | 0.048 | 0.883 | 1.000 | ||||||||||||||||||||||||||||||
| DDX42_G_f DDX42_G_r | 0.078 | 0.072 | 0.856 | 1.000 | ||||||||||||||||||||||||||||||
| DHX38_G_f DHX38_G_r | 1.000 | 1.000 | ||||||||||||||||||||||||||||||||
| HSPA2_G_3_f HSPA2_G_2_r | 0.056 | 0.062 | 0.549 | 1.000 | ||||||||||||||||||||||||||||||
| LUC7L_G_1_f LUC7L_G_r | 1.102 | 0.307 | 0.405 | 1.000 | ||||||||||||||||||||||||||||||
| MBNL2_G_1_f MBNL2_G_1_r | 0.850 | 0.775 | 0.099 | 1.000 |
current - show all
Page 1 of 1, records 1 to 8 of 8
QPCR Relative Expression Quantification (N1-3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N1)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N2)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Reference Gene Page (N1-3)
: .
QPCR Reference Gene Page (N1)
: .
QPCR Reference Gene Page (N2)
: .
QPCR Reference Gene Page (N3)
: .
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