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2. Breast Validation Screen 
Primer pair AS annotation : AS annotation
HSD17B1.F3 HSD17B1.R3

Targets alternative donor with size difference of 269
Short product(s): 231
Long product(s): 500
Isoform sequences

Targets exon skipping and alternative donor or acceptor with size difference of 97
Short product(s): 231
Long product(s): 328
Isoform sequences

Targets intron retention with size difference of 175
Short product(s): 325, 328
Long product(s): 500, 1017
Isoform sequences

Targets intron retention with size difference of 517
Short product(s): 325, 500
Long product(s): 1017
Isoform sequences

Targets intron retention with size difference of 514
Short product(s): 328
Long product(s): 1017
Isoform sequences

Targets intron retention with size difference of 786
Short product(s): 231
Long product(s): 1017
Isoform sequences

Targets single exon skipping with size difference of 94
Short product(s): 231
Long product(s): 325
Isoform sequences

Targets alternative acceptor with size difference of 3
Short product(s): 325, 500
Long product(s): 328
Isoform sequences

Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BDC 31188A1 / Well ID 954841
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BDC 50181A2 / Well ID 943225
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 121 / Well ID 942049
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 125 / Well ID 955224
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 129 / Well ID 944377
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 132 / Well ID 944737
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 138 / Well ID 942073
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 143 / Well ID 944761
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 149 / Well ID 943993
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 166 / Well ID 942433
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 169 / Well ID 945121
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 200 / Well ID 942457
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 206 / Well ID 955200
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 217 / Well ID 943585
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 222 / Well ID 943609
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 264 / Well ID 955201
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 269 / Well ID 943969
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 308 / Well ID 945145
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 328 / Well ID 945505
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 333 / Well ID 945529
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 364 / Well ID 942817
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 369 / Well ID 944353
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 375 / Well ID 942841
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 423 / Well ID 954817
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 426 / Well ID 943201
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRC 440 / Well ID 954840
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 1345 / Well ID 945504
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 1353 / Well ID 945528
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 138 / Well ID 942072
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 143 / Well ID 942432
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 158 / Well ID 942456
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 159 / Well ID 942816
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 166 / Well ID 942840
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 169 / Well ID 943200
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 172 / Well ID 943224
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 174 / Well ID 943584
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 187 / Well ID 954816
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 319 / Well ID 943608
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 345 / Well ID 943968
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 367 / Well ID 943992
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 423 / Well ID 944352
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 426 / Well ID 944376
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 492 / Well ID 944736
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 514 / Well ID 944760
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 517 / Well ID 945120
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN 58 / Well ID 942048
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / BRN F31 / Well ID 945144
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / HS 578T / Well ID 961859
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / MCF7 / Well ID 961834
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / MDA-MB-231 / Well ID 961858
Fluorescence graphs : HSD17B1.F3 / HSD17B1.R3 / ZR-75-1 / Well ID 961835

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