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2. Breast Validation Screen 
Transcript map : HLA-DQA1
Exon
Length: 92
Transcript: HLA-DQA1.aAug05
Exon
Length: 151
Transcript: HLA-DQA1.aAug05
Exon
Length: 166
Transcript: HLA-DQA1.aAug05
Exon
Length: 120
Transcript: HLA-DQA1.aAug05
Exon
Length: 249
Transcript: HLA-DQA1.aAug05
Exon
Length: 282
Transcript: HLA-DQA1.aAug05
Exon
Length: 175
Transcript: HLA-DQA1.aAug05
Exon
Length: 434
Transcript: HLA-DQA1.aAug05
Intron
Length: 3671
Transcript: HLA-DQA1.aAug05
Intron
Length: 651
Transcript: HLA-DQA1.aAug05
Intron
Length: 4511
Transcript: HLA-DQA1.aAug05
Intron
Length: 3769
Transcript: HLA-DQA1.aAug05
Intron
Length: 413
Transcript: HLA-DQA1.aAug05
Intron
Length: 356
Transcript: HLA-DQA1.aAug05
Intron
Length: 374
Transcript: HLA-DQA1.aAug05
Reaction
Expected peak: 241
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 288
Associated peak: 295,296,297,298
Repeated 51 time
Molarity: 12.89M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 143
Transcript: HLA-DQA1.bAug05
Exon
Length: 249
Transcript: HLA-DQA1.bAug05
Exon
Length: 282
Transcript: HLA-DQA1.bAug05
Exon
Length: 175
Transcript: HLA-DQA1.bAug05
Exon
Length: 2108
Transcript: HLA-DQA1.bAug05
Intron
Length: 3769
Transcript: HLA-DQA1.bAug05
Intron
Length: 413
Transcript: HLA-DQA1.bAug05
Intron
Length: 356
Transcript: HLA-DQA1.bAug05
Intron
Length: 167
Transcript: HLA-DQA1.bAug05
Reaction
Expected peak: 448
Associated peak: 459,460,461,464,466,467,468,469,470,471,472,473
Repeated 51 time
Molarity: 1.71M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 495
Associated peak: 512,518,519,524,525,526,529,530,531,532,533,534,535,538,539,540,545
Repeated 51 time
Molarity: 6.95M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 125
Transcript: HLA-DQA1.cAug05
Exon
Length: 6
Transcript: HLA-DQA1.cAug05
Exon
Length: 249
Transcript: HLA-DQA1.cAug05
Exon
Length: 282
Transcript: HLA-DQA1.cAug05
Exon
Length: 175
Transcript: HLA-DQA1.cAug05
Exon
Length: 360
Transcript: HLA-DQA1.cAug05
Intron
Length: 2808
Transcript: HLA-DQA1.cAug05
Intron
Length: 970
Transcript: HLA-DQA1.cAug05
Intron
Length: 413
Transcript: HLA-DQA1.cAug05
Intron
Length: 356
Transcript: HLA-DQA1.cAug05
Intron
Length: 508
Transcript: HLA-DQA1.cAug05
Reaction
Expected peak: 107
Associated peak: 113,114,115,116
Repeated 51 time
Molarity: 8.90M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Exon
Length: 120
Transcript: HLA-DQA1.dAug05
Exon
Length: 249
Transcript: HLA-DQA1.dAug05
Exon
Length: 5094
Transcript: HLA-DQA1.dAug05
Intron
Length: 3769
Transcript: HLA-DQA1.dAug05
Intron
Length: 413
Transcript: HLA-DQA1.dAug05
Reaction
Expected peak: 615
Associated peak: 624,627,631,633,635,638,641,642,647,652,653
Repeated 51 time
Molarity: 1.14M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 1018
Associated peak: 933,938,944,967
Repeated 51 time
Molarity: 0.14M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 117
Transcript: HLA-DQA1.eAug05
Exon
Length: 994
Transcript: HLA-DQA1.eAug05
Exon
Length: 249
Transcript: HLA-DQA1.eAug05
Exon
Length: 282
Transcript: HLA-DQA1.eAug05
Exon
Length: 175
Transcript: HLA-DQA1.eAug05
Exon
Length: 309
Transcript: HLA-DQA1.eAug05
Intron
Length: 1371
Transcript: HLA-DQA1.eAug05
Intron
Length: 1404
Transcript: HLA-DQA1.eAug05
Intron
Length: 413
Transcript: HLA-DQA1.eAug05
Intron
Length: 356
Transcript: HLA-DQA1.eAug05
Intron
Length: 167
Transcript: HLA-DQA1.eAug05
Reaction
Expected peak: 448
Associated peak: 459,460,461,464,466,467,468,469,470,471,472,473
Repeated 51 time
Molarity: 1.71M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 495
Associated peak: 512,518,519,524,525,526,529,530,531,532,533,534,535,538,539,540,545
Repeated 51 time
Molarity: 6.95M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 184
Transcript: HLA-DQA1.fAug05
Exon
Length: 249
Transcript: HLA-DQA1.fAug05
Exon
Length: 282
Transcript: HLA-DQA1.fAug05
Exon
Length: 30
Transcript: HLA-DQA1.fAug05
Intron
Length: 3769
Transcript: HLA-DQA1.fAug05
Intron
Length: 413
Transcript: HLA-DQA1.fAug05
Intron
Length: 698
Transcript: HLA-DQA1.fAug05
Reaction
Expected peak: 320
Associated peak: 309,311,312,314,315,331,332
Repeated 51 time
Molarity: 0.34M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 120
Transcript: HLA-DQA1.gAug05
Exon
Length: 249
Transcript: HLA-DQA1.gAug05
Exon
Length: 282
Transcript: HLA-DQA1.gAug05
Exon
Length: 97
Transcript: HLA-DQA1.gAug05
Intron
Length: 3769
Transcript: HLA-DQA1.gAug05
Intron
Length: 413
Transcript: HLA-DQA1.gAug05
Intron
Length: 905
Transcript: HLA-DQA1.gAug05
Reaction
Expected peak: 113
Associated peak: 124,126
Repeated 51 time
Molarity: 0.26M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 124
Transcript: HLA-DQA1.hAug05
Exon
Length: 249
Transcript: HLA-DQA1.hAug05
Exon
Length: 225
Transcript: HLA-DQA1.hAug05
Intron
Length: 3784
Transcript: HLA-DQA1.hAug05
Intron
Length: 413
Transcript: HLA-DQA1.hAug05
Reaction
Expected peak: 615
Associated peak: 624,627,631,633,635,638,641,642,647,652,653
Repeated 51 time
Molarity: 1.14M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 1018
Associated peak: 933,938,944,967
Repeated 51 time
Molarity: 0.14M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 115
Transcript: HLA-DQA1.iAug05
Exon
Length: 944
Transcript: HLA-DQA1.iAug05
Exon
Length: 294
Transcript: HLA-DQA1.iAug05
Intron
Length: 3769
Transcript: HLA-DQA1.iAug05
Intron
Length: 356
Transcript: HLA-DQA1.iAug05
Reaction
Expected peak: 615
Associated peak: 624,627,631,633,635,638,641,642,647,652,653
Repeated 51 time
Molarity: 1.14M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 662
Associated peak: 617
Repeated 51 time
Molarity: 0.04M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 109
Transcript: HLA-DQA1.jAug05
Exon
Length: 944
Transcript: HLA-DQA1.jAug05
Exon
Length: 155
Transcript: HLA-DQA1.jAug05
Exon
Length: 207
Transcript: HLA-DQA1.jAug05
Intron
Length: 3769
Transcript: HLA-DQA1.jAug05
Intron
Length: 356
Transcript: HLA-DQA1.jAug05
Intron
Length: 187
Transcript: HLA-DQA1.jAug05
Reaction
Expected peak: 428
Associated peak: 459,460,461,464,466,467,468,469,470,471,472,473
Repeated 51 time
Molarity: 1.71M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 475
Associated peak: 512,518,519,524,525,526,529,530,531,532,533,534,535
Repeated 51 time
Molarity: 5.70M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 109
Transcript: HLA-DQA1.kAug05
Exon
Length: 194
Transcript: HLA-DQA1.kAug05
Exon
Length: 282
Transcript: HLA-DQA1.kAug05
Exon
Length: 63
Transcript: HLA-DQA1.kAug05
Intron
Length: 3769
Transcript: HLA-DQA1.kAug05
Intron
Length: 468
Transcript: HLA-DQA1.kAug05
Intron
Length: 356
Transcript: HLA-DQA1.kAug05
Reaction
Expected peak: 615
Associated peak: 624,627,631,633,635,638,641,642,647,652,653
Repeated 51 time
Molarity: 1.14M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 662
Associated peak: 617
Repeated 51 time
Molarity: 0.04M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 120
Transcript: HLA-DQA1.lAug05
Exon
Length: 105
Transcript: HLA-DQA1.lAug05
Intron
Length: 4431
Transcript: HLA-DQA1.lAug05
Reaction
Expected peak: 615
Associated peak: 624,627,631,633,635,638,641,642,647,652,653
Repeated 51 time
Molarity: 1.14M
Forward: HLA-DQA1.F1
Reverse: HLA-DQA1.R1
Reaction
Expected peak: 1018
Associated peak: 933,938,944,967
Repeated 51 time
Molarity: 0.14M
Forward: HLA-DQA1.F3
Reverse: HLA-DQA1.R3
Exon
Length: 2374
Transcript: HLA-DQA1.shed1.aAug05
HLA-DQA1.R1
TTAGCCATAGTTTCAGTCAGCC
HLA-DQA1.F1
GCACTGTCTTCATCATCCAAGG
HLA-DQA1.F3
TTTATGACTGCAAGGTGGAGC
HLA-DQA1.R3
TACTGAGGAATTAGGTAGCCGG
Gene primer pair list : Primer pairs
Forward Reverse Occurences View
HLA-DQA1.F1 HLA-DQA1.R1 51 View
HLA-DQA1.F3 HLA-DQA1.R3 51 View

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