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2. Breast Validation Screen 
Primer pair AS annotation : AS annotation
HLA-DQA1.F3 HLA-DQA1.R3

Targets alternative donor with size difference of 20
Short product(s): 475
Long product(s): 495
Isoform sequences

Targets intron retention with size difference of 905
Short product(s): 113
Long product(s): 1018
Isoform sequences

Targets single exon skipping with size difference of 175
Short product(s): 320
Long product(s): 495
Isoform sequences

Targets alternative acceptor with size difference of 342
Short product(s): 320
Long product(s): 662
Isoform sequences

Targets intron retention with size difference of 167
Short product(s): 495
Long product(s): 662, 1018
Isoform sequences

Targets alternative acceptor with size difference of 549
Short product(s): 113
Long product(s): 662
Isoform sequences

Targets alternative acceptor with size difference of 207
Short product(s): 113
Long product(s): 320
Isoform sequences

Targets exon skipping and alternative donor or acceptor with size difference of 32
Short product(s): 288
Long product(s): 320
Isoform sequences

Targets alternative donor and acceptor with size difference of 187
Short product(s): 288
Long product(s): 475
Isoform sequences

Targets intron retention with size difference of 698
Short product(s): 320
Long product(s): 1018
Isoform sequences

Targets intron retention with size difference of 356
Short product(s): 288, 475, 495, 662
Long product(s): 1018
Isoform sequences

Targets intron retention with size difference of 187
Short product(s): 475
Long product(s): 662, 1018
Isoform sequences

Targets exon skipping and alternative donor or acceptor with size difference of 382
Short product(s): 113
Long product(s): 495
Isoform sequences

Targets alternative acceptor with size difference of 207
Short product(s): 288
Long product(s): 495
Isoform sequences

Targets single exon skipping with size difference of 175
Short product(s): 113
Long product(s): 288
Isoform sequences

Targets exon skipping and alternative donor or acceptor with size difference of 362
Short product(s): 113
Long product(s): 475
Isoform sequences

Targets intron retention with size difference of 374
Short product(s): 288
Long product(s): 662, 1018
Isoform sequences

Targets single exon skipping with size difference of 155
Short product(s): 320
Long product(s): 475
Isoform sequences

Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BDC 31188A1 / Well ID 955085
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BDC 50181A2 / Well ID 943469
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 121 / Well ID 942293
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 125 / Well ID 955468
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 129 / Well ID 944621
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 132 / Well ID 944981
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 138 / Well ID 942317
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 143 / Well ID 945005
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 149 / Well ID 944237
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 166 / Well ID 942677
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 169 / Well ID 945365
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 200 / Well ID 942701
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 206 / Well ID 955444
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 217 / Well ID 943829
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 222 / Well ID 943853
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 264 / Well ID 955445
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 269 / Well ID 944213
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 308 / Well ID 945389
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 328 / Well ID 945749
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 333 / Well ID 945773
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 364 / Well ID 943061
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 369 / Well ID 944597
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 375 / Well ID 943085
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 423 / Well ID 955061
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 426 / Well ID 943445
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRC 440 / Well ID 955084
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 1345 / Well ID 945748
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 1353 / Well ID 945772
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 138 / Well ID 942316
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 143 / Well ID 942676
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 158 / Well ID 942700
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 159 / Well ID 943060
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 166 / Well ID 943084
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 169 / Well ID 943444
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 172 / Well ID 943468
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 174 / Well ID 943828
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 187 / Well ID 955060
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 319 / Well ID 943852
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 345 / Well ID 944212
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 367 / Well ID 944236
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 423 / Well ID 944596
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 426 / Well ID 944620
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 492 / Well ID 944980
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 514 / Well ID 945004
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 517 / Well ID 945364
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN 58 / Well ID 942292
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / BRN F31 / Well ID 945388
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / HS 578T / Well ID 962103
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / MCF7 / Well ID 962078
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / MDA-MB-231 / Well ID 962102
Fluorescence graphs : HLA-DQA1.F3 / HLA-DQA1.R3 / ZR-75-1 / Well ID 962079

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