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Supplementary data
Tumor microenvironment associated modifications of alternative splicing
Splicing factor knockdown
Validation
RBM15 RBM4B RBM5 RBMS3 RPS13 SART1 SSBP3
QPCR Primer Pair List
: primer pair list
| Gene | Forward | Reverse | |
|---|---|---|---|
| VIEW | PSMC4 | PSMC4_G_1_f | PSMC4_G_1_r |
| VIEW | RBM15 | RBM15_G_2_f | RBM15_G_2_r |
| VIEW | RBM4B | RBM4B.G1.F1 | RBM4B.G1.R1 |
| VIEW | RBM5 | RBM5_G_1_f | RBM5_G_r |
| VIEW | RBMS3 | RBMS3_G_f | RBMS3_G_r |
| VIEW | RPS13 | RPS13_G_f | RPS13_G_r |
| VIEW | SART1 | SART1_G_f | SART1_G_r |
| VIEW | SDHA | SDHA_G_f | SDHA_G_r |
| VIEW | SSBP3 | SSBP3_G_f | SSBP3_G_r |
current - show all
Page 1 of 1, records 1 to 9 of 9
QPCR Relative Expression Quantification (N1-3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | KITLG_84_L_2_s;SKOV3ip1 |
| 2 | RBM15_RBP_G_s;SKOV3ip1 |
| 3 | RBM15__G_s;SKOV3ip1 |
| 4 | RBM4B__G_1_s;SKOV3ip1 |
| 5 | RBM4B__G_s;SKOV3ip1 |
| 6 | RBM5__G_s;SKOV3ip1 |
| 7 | RBM5_global_1;SKOV3ip1 |
| 8 | RBMS3__G_1_s;SKOV3ip1 |
| 9 | RBMS3__G_s;SKOV3ip1 |
| 10 | RPS13__G_1_s;SKOV3ip1 |
| 11 | RPS13__G_s;SKOV3ip1 |
| 12 | SART1__G_1_s;SKOV3ip1 |
| 13 | SART1__G_s;SKOV3ip1 |
| 14 | SKOV3ip1;Lipofectamine 2000 |
| 15 | SMNDC1__G_1_s;SKOV3ip1 |
| 16 | SMNDC1__G_s;SKOV3ip1 |
| 17 | SSBP3__G_2_s;SKOV3ip1 |
| 18 | SSBP3__G_3_s;SKOV3ip1 |
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Page 1 of 1, records 1 to 18 of 18
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 | S18 | p18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RBM15_G_2_f RBM15_G_2_r | 0.554 | 0.299 | 1.000 | |||||||||||||||||||||||||||||||||
| RBM4B.G1.F1 RBM4B.G1.R1 | 1.143 | 0.458 | 0.426 | 1.000 | ||||||||||||||||||||||||||||||||
| RBM5_G_1_f RBM5_G_r | 1.059 | 0.332 | 0.148 | 1.000 | ||||||||||||||||||||||||||||||||
| RBMS3_G_f RBMS3_G_r | 1.422 | 0.363 | 1.000 | |||||||||||||||||||||||||||||||||
| RPS13_G_f RPS13_G_r | 1.359 | 0.052 | 0.046 | 1.000 | ||||||||||||||||||||||||||||||||
| SART1_G_f SART1_G_r | 1.033 | 0.275 | 0.112 | 1.000 | ||||||||||||||||||||||||||||||||
| SSBP3_G_f SSBP3_G_r | 1.104 | 1.000 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N1)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | KITLG_84_L_2_s;SKOV3ip1 |
| 2 | RBM15_RBP_G_s;SKOV3ip1 |
| 3 | RBM15__G_s;SKOV3ip1 |
| 4 | RBM4B__G_1_s;SKOV3ip1 |
| 5 | RBM4B__G_s;SKOV3ip1 |
| 6 | RBM5__G_s;SKOV3ip1 |
| 7 | RBM5_global_1;SKOV3ip1 |
| 8 | RBMS3__G_1_s;SKOV3ip1 |
| 9 | RBMS3__G_s;SKOV3ip1 |
| 10 | RPS13__G_1_s;SKOV3ip1 |
| 11 | RPS13__G_s;SKOV3ip1 |
| 12 | SART1__G_1_s;SKOV3ip1 |
| 13 | SART1__G_s;SKOV3ip1 |
| 14 | SKOV3ip1;Lipofectamine 2000 |
| 15 | SMNDC1__G_1_s;SKOV3ip1 |
| 16 | SMNDC1__G_s;SKOV3ip1 |
| 17 | SSBP3__G_2_s;SKOV3ip1 |
| 18 | SSBP3__G_3_s;SKOV3ip1 |
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Page 1 of 1, records 1 to 18 of 18
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 | S18 | p18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RBM15_G_2_f RBM15_G_2_r | 0.554 | 0.299 | 1.000 | |||||||||||||||||||||||||||||||||
| RBM4B.G1.F1 RBM4B.G1.R1 | 1.143 | 0.458 | 0.426 | 1.000 | ||||||||||||||||||||||||||||||||
| RBM5_G_1_f RBM5_G_r | 1.059 | 0.332 | 0.148 | 1.000 | ||||||||||||||||||||||||||||||||
| RBMS3_G_f RBMS3_G_r | 1.422 | 0.363 | 1.000 | |||||||||||||||||||||||||||||||||
| RPS13_G_f RPS13_G_r | 1.359 | 0.052 | 0.046 | 1.000 | ||||||||||||||||||||||||||||||||
| SART1_G_f SART1_G_r | 1.033 | 0.275 | 0.112 | 1.000 | ||||||||||||||||||||||||||||||||
| SSBP3_G_f SSBP3_G_r | 1.104 | 1.000 |
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Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N2)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | KITLG_84_L_2_s;SKOV3ip1 |
| 2 | RBM15_RBP_G_s;SKOV3ip1 |
| 3 | RBM15__G_s;SKOV3ip1 |
| 4 | RBM4B__G_1_s;SKOV3ip1 |
| 5 | RBM4B__G_s;SKOV3ip1 |
| 6 | RBM5__G_s;SKOV3ip1 |
| 7 | RBM5_global_1;SKOV3ip1 |
| 8 | RBMS3__G_1_s;SKOV3ip1 |
| 9 | RBMS3__G_s;SKOV3ip1 |
| 10 | RPS13__G_1_s;SKOV3ip1 |
| 11 | RPS13__G_s;SKOV3ip1 |
| 12 | SART1__G_1_s;SKOV3ip1 |
| 13 | SART1__G_s;SKOV3ip1 |
| 14 | SKOV3ip1;Lipofectamine 2000 |
| 15 | SMNDC1__G_1_s;SKOV3ip1 |
| 16 | SMNDC1__G_s;SKOV3ip1 |
| 17 | SSBP3__G_2_s;SKOV3ip1 |
| 18 | SSBP3__G_3_s;SKOV3ip1 |
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Page 1 of 1, records 1 to 18 of 18
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 | S18 | p18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RBM15_G_2_f RBM15_G_2_r | 0.554 | 0.299 | 1.000 | |||||||||||||||||||||||||||||||||
| RBM4B.G1.F1 RBM4B.G1.R1 | 1.143 | 0.458 | 0.426 | 1.000 | ||||||||||||||||||||||||||||||||
| RBM5_G_1_f RBM5_G_r | 1.059 | 0.332 | 0.148 | 1.000 | ||||||||||||||||||||||||||||||||
| RBMS3_G_f RBMS3_G_r | 1.422 | 0.363 | 1.000 | |||||||||||||||||||||||||||||||||
| RPS13_G_f RPS13_G_r | 1.359 | 0.052 | 0.046 | 1.000 | ||||||||||||||||||||||||||||||||
| SART1_G_f SART1_G_r | 1.033 | 0.275 | 0.112 | 1.000 | ||||||||||||||||||||||||||||||||
| SSBP3_G_f SSBP3_G_r | 1.104 | 1.000 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N3)
: Summary
Expression Table
| ID | Name |
|---|---|
| 1 | KITLG_84_L_2_s;SKOV3ip1 |
| 2 | RBM15_RBP_G_s;SKOV3ip1 |
| 3 | RBM15__G_s;SKOV3ip1 |
| 4 | RBM4B__G_1_s;SKOV3ip1 |
| 5 | RBM4B__G_s;SKOV3ip1 |
| 6 | RBM5__G_s;SKOV3ip1 |
| 7 | RBM5_global_1;SKOV3ip1 |
| 8 | RBMS3__G_1_s;SKOV3ip1 |
| 9 | RBMS3__G_s;SKOV3ip1 |
| 10 | RPS13__G_1_s;SKOV3ip1 |
| 11 | RPS13__G_s;SKOV3ip1 |
| 12 | SART1__G_1_s;SKOV3ip1 |
| 13 | SART1__G_s;SKOV3ip1 |
| 14 | SKOV3ip1;Lipofectamine 2000 |
| 15 | SMNDC1__G_1_s;SKOV3ip1 |
| 16 | SMNDC1__G_s;SKOV3ip1 |
| 17 | SSBP3__G_2_s;SKOV3ip1 |
| 18 | SSBP3__G_3_s;SKOV3ip1 |
current - show all
Page 1 of 1, records 1 to 18 of 18
| Primer Pair | S1 | p1 | S2 | p2 | S3 | p3 | S4 | p4 | S5 | p5 | S6 | p6 | S7 | p7 | S8 | p8 | S9 | p9 | S10 | p10 | S11 | p11 | S12 | p12 | S13 | p13 | S14 | p14 | S15 | p15 | S16 | p16 | S17 | p17 | S18 | p18 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RBM15_G_2_f RBM15_G_2_r | 0.554 | 0.299 | 1.000 | |||||||||||||||||||||||||||||||||
| RBM4B.G1.F1 RBM4B.G1.R1 | 1.143 | 0.458 | 0.426 | 1.000 | ||||||||||||||||||||||||||||||||
| RBM5_G_1_f RBM5_G_r | 1.059 | 0.332 | 0.148 | 1.000 | ||||||||||||||||||||||||||||||||
| RBMS3_G_f RBMS3_G_r | 1.422 | 0.363 | 1.000 | |||||||||||||||||||||||||||||||||
| RPS13_G_f RPS13_G_r | 1.359 | 0.052 | 0.046 | 1.000 | ||||||||||||||||||||||||||||||||
| SART1_G_f SART1_G_r | 1.033 | 0.275 | 0.112 | 1.000 | ||||||||||||||||||||||||||||||||
| SSBP3_G_f SSBP3_G_r | 1.104 | 1.000 |
current - show all
Page 1 of 1, records 1 to 7 of 7
QPCR Relative Expression Quantification (N1-3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N1)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N2)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Relative Expression Quantification (N3)
: Systematic Outlier Filtering
0 technical replicates removed (absolute Ct threshold 0.75; MADZ 3.5)
Click here to view
0 biological replicates removed (relative expression threshold 0.25; MADZ 2.1)
Click here to view
QPCR Reference Gene Page (N1-3)
: .
QPCR Reference Gene Page (N1)
: .
QPCR Reference Gene Page (N2)
: .
QPCR Reference Gene Page (N3)
: .
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