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Identification of alternative splicing markers for breast cancer
2. Breast Validation Screen
Transcript map
: HSD17B1
Exon
Length: 3817
Transcript: HSD17B1.aAug05
Length: 3817
Transcript: HSD17B1.aAug05
Exon
Length: 168
Transcript: HSD17B1.aAug05
Length: 168
Transcript: HSD17B1.aAug05
Exon
Length: 180
Transcript: HSD17B1.aAug05
Length: 180
Transcript: HSD17B1.aAug05
Exon
Length: 94
Transcript: HSD17B1.aAug05
Length: 94
Transcript: HSD17B1.aAug05
Exon
Length: 178
Transcript: HSD17B1.aAug05
Length: 178
Transcript: HSD17B1.aAug05
Exon
Length: 547
Transcript: HSD17B1.aAug05
Length: 547
Transcript: HSD17B1.aAug05
Intron
Length: 93
Transcript: HSD17B1.aAug05
Length: 93
Transcript: HSD17B1.aAug05
Intron
Length: 147
Transcript: HSD17B1.aAug05
Length: 147
Transcript: HSD17B1.aAug05
Intron
Length: 175
Transcript: HSD17B1.aAug05
Length: 175
Transcript: HSD17B1.aAug05
Intron
Length: 517
Transcript: HSD17B1.aAug05
Length: 517
Transcript: HSD17B1.aAug05
Intron
Length: 86
Transcript: HSD17B1.aAug05
Length: 86
Transcript: HSD17B1.aAug05
Reaction
Expected peak: 307
Associated peak: 318,319,320,321,322,323,324
Repeated 51 time
Molarity: 6.28M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 307
Associated peak: 318,319,320,321,322,323,324
Repeated 51 time
Molarity: 6.28M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 325
Associated peak: 344,346,347,348,349,350,351,352,353
Repeated 51 time
Molarity: 4.07M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 325
Associated peak: 344,346,347,348,349,350,351,352,353
Repeated 51 time
Molarity: 4.07M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 98
Transcript: HSD17B1.bAug05
Length: 98
Transcript: HSD17B1.bAug05
Exon
Length: 168
Transcript: HSD17B1.bAug05
Length: 168
Transcript: HSD17B1.bAug05
Exon
Length: 180
Transcript: HSD17B1.bAug05
Length: 180
Transcript: HSD17B1.bAug05
Exon
Length: 94
Transcript: HSD17B1.bAug05
Length: 94
Transcript: HSD17B1.bAug05
Exon
Length: 181
Transcript: HSD17B1.bAug05
Length: 181
Transcript: HSD17B1.bAug05
Exon
Length: 516
Transcript: HSD17B1.bAug05
Length: 516
Transcript: HSD17B1.bAug05
Intron
Length: 93
Transcript: HSD17B1.bAug05
Length: 93
Transcript: HSD17B1.bAug05
Intron
Length: 147
Transcript: HSD17B1.bAug05
Length: 147
Transcript: HSD17B1.bAug05
Intron
Length: 175
Transcript: HSD17B1.bAug05
Length: 175
Transcript: HSD17B1.bAug05
Intron
Length: 514
Transcript: HSD17B1.bAug05
Length: 514
Transcript: HSD17B1.bAug05
Intron
Length: 86
Transcript: HSD17B1.bAug05
Length: 86
Transcript: HSD17B1.bAug05
Reaction
Expected peak: 310
Associated peak: 318,319,320,321,322,323,324
Repeated 51 time
Molarity: 6.28M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 310
Associated peak: 318,319,320,321,322,323,324
Repeated 51 time
Molarity: 6.28M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 328
Associated peak: 344,346,347,348,349,350,351,352,353
Repeated 51 time
Molarity: 4.07M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 328
Associated peak: 344,346,347,348,349,350,351,352,353
Repeated 51 time
Molarity: 4.07M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 435
Transcript: HSD17B1.cAug05
Length: 435
Transcript: HSD17B1.cAug05
Exon
Length: 521
Transcript: HSD17B1.cAug05
Length: 521
Transcript: HSD17B1.cAug05
Intron
Length: 86
Transcript: HSD17B1.cAug05
Length: 86
Transcript: HSD17B1.cAug05
Reaction
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 370
Transcript: HSD17B1.dAug05
Length: 370
Transcript: HSD17B1.dAug05
Exon
Length: 449
Transcript: HSD17B1.dAug05
Length: 449
Transcript: HSD17B1.dAug05
Exon
Length: 178
Transcript: HSD17B1.dAug05
Length: 178
Transcript: HSD17B1.dAug05
Exon
Length: 377
Transcript: HSD17B1.dAug05
Length: 377
Transcript: HSD17B1.dAug05
Intron
Length: 147
Transcript: HSD17B1.dAug05
Length: 147
Transcript: HSD17B1.dAug05
Intron
Length: 517
Transcript: HSD17B1.dAug05
Length: 517
Transcript: HSD17B1.dAug05
Intron
Length: 86
Transcript: HSD17B1.dAug05
Length: 86
Transcript: HSD17B1.dAug05
Reaction
Expected peak: 482
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 482
Associated peak: None
Repeated 51 time
Molarity: 0.00M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 500
Associated peak: 470,472,478,480,482,484,486
Repeated 51 time
Molarity: 0.15M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 500
Associated peak: 470,472,478,480,482,484,486
Repeated 51 time
Molarity: 0.15M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 108
Transcript: HSD17B1.eAug05
Length: 108
Transcript: HSD17B1.eAug05
Exon
Length: 168
Transcript: HSD17B1.eAug05
Length: 168
Transcript: HSD17B1.eAug05
Exon
Length: 700
Transcript: HSD17B1.eAug05
Length: 700
Transcript: HSD17B1.eAug05
Intron
Length: 93
Transcript: HSD17B1.eAug05
Length: 93
Transcript: HSD17B1.eAug05
Intron
Length: 147
Transcript: HSD17B1.eAug05
Length: 147
Transcript: HSD17B1.eAug05
Reaction
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 106
Transcript: HSD17B1.fAug05
Length: 106
Transcript: HSD17B1.fAug05
Exon
Length: 168
Transcript: HSD17B1.fAug05
Length: 168
Transcript: HSD17B1.fAug05
Exon
Length: 180
Transcript: HSD17B1.fAug05
Length: 180
Transcript: HSD17B1.fAug05
Exon
Length: 35
Transcript: HSD17B1.fAug05
Length: 35
Transcript: HSD17B1.fAug05
Intron
Length: 93
Transcript: HSD17B1.fAug05
Length: 93
Transcript: HSD17B1.fAug05
Intron
Length: 147
Transcript: HSD17B1.fAug05
Length: 147
Transcript: HSD17B1.fAug05
Intron
Length: 786
Transcript: HSD17B1.fAug05
Length: 786
Transcript: HSD17B1.fAug05
Reaction
Expected peak: 213
Associated peak: 229,230,231,232,233,240
Repeated 51 time
Molarity: 3.97M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 213
Associated peak: 229,230,231,232,233,240
Repeated 51 time
Molarity: 3.97M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 231
Associated peak: 249,250,251,252,253
Repeated 51 time
Molarity: 2.31M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 231
Associated peak: 249,250,251,252,253
Repeated 51 time
Molarity: 2.31M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 60
Transcript: HSD17B1.gAug05
Length: 60
Transcript: HSD17B1.gAug05
Exon
Length: 932
Transcript: HSD17B1.gAug05
Length: 932
Transcript: HSD17B1.gAug05
Intron
Length: 93
Transcript: HSD17B1.gAug05
Length: 93
Transcript: HSD17B1.gAug05
Reaction
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Exon
Length: 2856
Transcript: HSD17B1.shed1.aAug05
Length: 2856
Transcript: HSD17B1.shed1.aAug05
Reaction
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Expected peak: 999
Associated peak: 905,963,967,974,977,981,984,989,992,995,1000,1011
Repeated 51 time
Molarity: 2.12M
Forward: HSD17B1.F4
Reverse: HSD17B1.R4
Reaction
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
Expected peak: 1017
Associated peak: 995,997,1014,1016,1018,1024,1026
Repeated 51 time
Molarity: 0.65M
Forward: HSD17B1.F3
Reverse: HSD17B1.R3
HSD17B1.F4
CTTCCTGCCAGACATGAAGAG
CTTCCTGCCAGACATGAAGAG
HSD17B1.F3
TGCTGGACGTGAATGTAGTAGG
TGCTGGACGTGAATGTAGTAGG
HSD17B1.R4
AAAGACTTGCTTGCTGTGGG
AAAGACTTGCTTGCTGTGGG
HSD17B1.R3
TATTGGTAGAAGCGGTGGAAGG
TATTGGTAGAAGCGGTGGAAGG