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2. Breast Validation Screen 
Primer pair AS annotation : AS annotation
HSD17B1.F4 HSD17B1.R4

Targets alternative donor with size difference of 269
Short product(s): 213
Long product(s): 482
Isoform sequences

Targets exon skipping and alternative donor or acceptor with size difference of 97
Short product(s): 213
Long product(s): 310
Isoform sequences

Targets intron retention with size difference of 175
Short product(s): 307, 310
Long product(s): 482, 999
Isoform sequences

Targets intron retention with size difference of 517
Short product(s): 307, 482
Long product(s): 999
Isoform sequences

Targets intron retention with size difference of 514
Short product(s): 310
Long product(s): 999
Isoform sequences

Targets intron retention with size difference of 786
Short product(s): 213
Long product(s): 999
Isoform sequences

Targets single exon skipping with size difference of 94
Short product(s): 213
Long product(s): 307
Isoform sequences

Targets alternative acceptor with size difference of 3
Short product(s): 307, 482
Long product(s): 310
Isoform sequences

Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BDC 31188A1 / Well ID 954839
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BDC 50181A2 / Well ID 943223
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 121 / Well ID 942047
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 125 / Well ID 955222
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 129 / Well ID 944375
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 132 / Well ID 944735
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 138 / Well ID 942071
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 143 / Well ID 944759
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 149 / Well ID 943991
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 166 / Well ID 942431
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 169 / Well ID 945119
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 200 / Well ID 942455
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 206 / Well ID 955198
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 217 / Well ID 943583
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 222 / Well ID 943607
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 264 / Well ID 955199
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 269 / Well ID 943967
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 308 / Well ID 945143
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 328 / Well ID 945503
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 333 / Well ID 945527
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 364 / Well ID 942815
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 369 / Well ID 944351
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 375 / Well ID 942839
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 423 / Well ID 954815
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 426 / Well ID 943199
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRC 440 / Well ID 954838
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 1345 / Well ID 945502
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 1353 / Well ID 945526
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 138 / Well ID 942070
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 143 / Well ID 942430
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 158 / Well ID 942454
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 159 / Well ID 942814
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 166 / Well ID 942838
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 169 / Well ID 943198
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 172 / Well ID 943222
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 174 / Well ID 943582
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 187 / Well ID 954814
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 319 / Well ID 943606
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 345 / Well ID 943966
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 367 / Well ID 943990
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 423 / Well ID 944350
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 426 / Well ID 944374
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 492 / Well ID 944734
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 514 / Well ID 944758
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 517 / Well ID 945118
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN 58 / Well ID 942046
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / BRN F31 / Well ID 945142
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / HS 578T / Well ID 961857
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / MCF7 / Well ID 961832
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / MDA-MB-231 / Well ID 961856
Fluorescence graphs : HSD17B1.F4 / HSD17B1.R4 / ZR-75-1 / Well ID 961833

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